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. 2000 Oct;66(10):4555-8.
doi: 10.1128/AEM.66.10.4555-4558.2000.

Rapid and simple determination of the Escherichia coli phylogenetic group

O Clermont  1 , S Bonacorsi, E Bingen
Affiliations

Rapid and simple determination of the Escherichia coli phylogenetic group

O Clermont et al. Appl Environ Microbiol. 2000 Oct.

Abstract

Phylogenetic analysis has shown that Escherichia coli is composed of four main phylogenetic groups (A, B1, B2, and D) and that virulent extra-intestinal strains mainly belong to groups B2 and D. Actually, phylogenetic groups can be determined by multilocus enzyme electrophoresis or ribotyping, both of which are complex, time-consuming techniques. We describe a simple and rapid phylogenetic grouping technique based on triplex PCR. The method, which uses a combination of two genes (chuA and yjaA) and an anonymous DNA fragment, was tested with 230 strains and showed excellent correlation with reference methods.

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Figures

FIG. 1
FIG. 1
Dichotomous decision tree to determine the phylogenetic group of an E. coli strain by using the results of PCR amplification of the chuA and yjaA genes and DNA fragment TSPE4.C2.
FIG. 2
FIG. 2
Triplex PCR profiles specific for E. coli phylogenetic groups. Each combination of chuA and yjaA gene and DNA fragment TSPE4.C2 amplification allowed phylogenetic group determination of a strain. Lanes 1 and 2, group A; lane 3, group B1; lanes 4 and 5, group D; lanes 6 and 7, group B2. Lane M contained markers.
See this image and copyright information in PMC

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