Regulation of glycogen synthesis by amino acids in cultured human muscle cells
- PMID: 11013237
- DOI: 10.1074/jbc.M004812200
Regulation of glycogen synthesis by amino acids in cultured human muscle cells
Abstract
Insulin and a number of metabolic factors stimulate glycogen synthesis and the enzyme glycogen synthase. Using human muscle cells we find that glycogen synthesis is stimulated by treatment of the cells with lithium ions, which inhibit glycogen synthase kinase 3. Insulin further stimulates glycogen synthesis in the presence of lithium ions, an effect abolished by wortmannin and rapamycin. We report also that amino acids stimulate glycogen synthesis and glycogen synthase, these effects also being blocked by rapamycin and wortmannin. Amino acids stimulate p70(s6k) and transiently inhibit glycogen synthase kinase 3 without effects on the activity of protein kinase B or the mitogen-activated protein kinase pathway. Thus, the work reported here demonstrates that amino acid availability can regulate glycogen synthesis. Furthermore, it demonstrates that glycogen synthase kinase 3 can be inactivated within cells independent of activation of protein kinase B and p90(rsk).
Similar articles
-
L-leucine availability regulates phosphatidylinositol 3-kinase, p70 S6 kinase and glycogen synthase kinase-3 activity in L6 muscle cells: evidence for the involvement of the mammalian target of rapamycin (mTOR) pathway in the L-leucine-induced up-regulation of system A amino acid transport.Biochem J. 2000 Sep 1;350 Pt 2(Pt 2):361-8. Biochem J. 2000. PMID: 10947949 Free PMC article.
-
The inhibition of glycogen synthase kinase-3 by insulin or insulin-like growth factor 1 in the rat skeletal muscle cell line L6 is blocked by wortmannin, but not by rapamycin: evidence that wortmannin blocks activation of the mitogen-activated protein kinase pathway in L6 cells between Ras and Raf.Biochem J. 1994 Oct 1;303 ( Pt 1)(Pt 1):21-6. doi: 10.1042/bj3030021. Biochem J. 1994. PMID: 7945242 Free PMC article.
-
Reciprocal regulation of glycogen phosphorylase and glycogen synthase by insulin involving phosphatidylinositol-3 kinase and protein phosphatase-1 in HepG2 cells.Mol Cell Biochem. 2000 Aug;211(1-2):123-36. doi: 10.1023/a:1007159422667. Mol Cell Biochem. 2000. PMID: 11055555
-
Multiple signalling pathways involved in the stimulation of fatty acid and glycogen synthesis by insulin in rat epididymal fat cells.Biochem J. 1995 Oct 15;311 ( Pt 2)(Pt 2):595-601. doi: 10.1042/bj3110595. Biochem J. 1995. PMID: 7487901 Free PMC article.
-
The role of glycogen synthase kinase 3beta in insulin-stimulated glucose metabolism.J Biol Chem. 1999 Jun 18;274(25):17934-40. doi: 10.1074/jbc.274.25.17934. J Biol Chem. 1999. PMID: 10364240
Cited by
-
S6K1 regulates GSK3 under conditions of mTOR-dependent feedback inhibition of Akt.Mol Cell. 2006 Oct 20;24(2):185-97. doi: 10.1016/j.molcel.2006.09.019. Mol Cell. 2006. PMID: 17052453 Free PMC article.
-
The Role of Glycogen Synthase Kinase 3 Beta in Neuroinflammation and Pain.J Pharm Pharmacol (Los Angel). 2013;1(1):001. doi: 10.13188/2327-204X.1000001. J Pharm Pharmacol (Los Angel). 2013. PMID: 25309941 Free PMC article.
-
Parallel comparative proteomics and phosphoproteomics reveal that cattle myostatin regulates phosphorylation of key enzymes in glycogen metabolism and glycolysis pathway.Oncotarget. 2018 Jan 13;9(13):11352-11370. doi: 10.18632/oncotarget.24250. eCollection 2018 Feb 16. Oncotarget. 2018. PMID: 29541418 Free PMC article.
-
White Adipocyte Stem Cell Expansion Through Infant Formula Feeding: New Insights into Epigenetic Programming Explaining the Early Protein Hypothesis of Obesity.Int J Mol Sci. 2025 May 8;26(10):4493. doi: 10.3390/ijms26104493. Int J Mol Sci. 2025. PMID: 40429638 Free PMC article. Review.
-
Branched-chain amino acid supplementation: impact on signaling and relevance to critical illness.Wiley Interdiscip Rev Syst Biol Med. 2013 Jul-Aug;5(4):449-460. doi: 10.1002/wsbm.1219. Epub 2013 Mar 29. Wiley Interdiscip Rev Syst Biol Med. 2013. PMID: 23554299 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources