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Multicenter Study
. 2000 Oct;38(10):3527-33.
doi: 10.1128/JCM.38.10.3527-3533.2000.

Multicenter evaluation of epidemiological typing of methicillin-resistant Staphylococcus aureus strains by repetitive-element PCR analysis. The European Study Group on Epidemiological Markers of the ESCMID

Affiliations
Multicenter Study

Multicenter evaluation of epidemiological typing of methicillin-resistant Staphylococcus aureus strains by repetitive-element PCR analysis. The European Study Group on Epidemiological Markers of the ESCMID

A Deplano et al. J Clin Microbiol. 2000 Oct.

Abstract

Rapid and efficient epidemiologic typing systems would be useful to monitor transmission of methicillin-resistant Staphylococcus aureus (MRSA) at both local and interregional levels. To evaluate the intralaboratory performance and interlaboratory reproducibility of three recently developed repeat-element PCR (rep-PCR) methods for the typing of MRSA, 50 MRSA strains characterized by pulsed-field gel electrophoresis (PFGE) (SmaI) analysis and epidemiological data were blindly typed by inter-IS256, 16S-23S ribosomal DNA (rDNA), and MP3 PCR in 12 laboratories in eight countries using standard reagents and protocols. Performance of typing was defined by reproducibility (R), discriminatory power (D), and agreement with PFGE analysis. Interlaboratory reproducibility of pattern and type classification was assessed visually and using gel analysis software. Each typing method showed a different performance level in each center. In the center performing best with each method, inter-IS256 PCR typing achieved R = 100% and D = 100%; 16S-23S rDNA PCR, R = 100% and D = 82%; and MP3 PCR, R = 80% and D = 83%. Concordance between rep-PCR type and PFGE type ranged by center: 70 to 90% for inter-IS256 PCR, 44 to 57% for 16S-23S rDNA PCR, and 53 to 54% for MP3 PCR analysis. In conclusion, the performance of inter-IS256 PCR typing was similar to that of PFGE analysis in some but not all centers, whereas other rep-PCR protocols showed lower discrimination and intralaboratory reproducibility. None of these assays, however, was sufficiently reproducible for interlaboratory exchange of data.

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Figures

FIG. 1
FIG. 1
Patterns, clustering, and type classification of the 45 MRSA strains by PFGE analysis and inter-IS256, 16S-23S rRNA, and MP3 PCR patterns resolved by agarose electrophoresis in the center obtaining the best performance with each method.
FIG. 2
FIG. 2
Dendrogram of similarity of MRSA strains based on ALFA of inter-IS256 PCR, 16S-23S rRNA PCR, and MP3 PCR patterns produced by laboratories with optimal performance score compared with classification in PFGE type. The cut-off value for type identity was based on mean duplicate similarity values (Table 4).

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