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Comparative Study
. 2000 Oct;38(10):3843-5.
doi: 10.1128/JCM.38.10.3843-3845.2000.

Use of amplified fragment length polymorphisms for typing Corynebacterium diphtheriae

A De Zoysa  1 , A Efstratiou
Affiliations
Comparative Study

Use of amplified fragment length polymorphisms for typing Corynebacterium diphtheriae

A De Zoysa et al. J Clin Microbiol. 2000 Oct.

Abstract

Amplified fragment length polymorphism (AFLP) was investigated for the differentiation of Corynebacterium diphtheriae isolates. Analysis using Taxotron revealed 10 distinct AFLP profiles among 57 isolates. Strains with ribotype patterns D1, D4, and D12 could not be distinguished; however, the technique discriminated isolates of ribotype patterns D3, D6, and D7 further. AFLP was rapid, fairly inexpensive, and reproducible and could be used as an alternative to ribotyping.

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Figures

FIG. 1
FIG. 1
AFLP profiles of C. diphtheriae produced by primer PstI-G. Lanes 1, 4, 7, 10, 13, 16, and 19, 100-bp molecular weight standard (with sizes indicated on the left). The remaining lanes show AFLP profiles of the following strains: C93/46 (lane 2), C95/66 (lane 3), C95/87 (lane 5), C93/69 (lane 6), C93/78 (lane 8), C93/266 (lane 9), C95/115 (lane 11), C93/181 (lane 12), C95/59 (lane 14), C93/45 (lane 15), C93/274 (lane 17), and C93/277 (lane 18). AFLP types are given above each lane, and ribotype designations are given at the bottom of each lane.
FIG. 2
FIG. 2
Cluster analysis of the AFLP profiles of the C. diphtheriae strains shown in Fig. 1. The strain numbers, AFLP types, and ribotype designations are given on the right. Analyses were performed with the Taxotron software package (Institut Pasteur). Patterns were clustered by the single-linkage method with a fixed tolerance of 4%. A genetic distance of zero is equal to 100% similarity.
See this image and copyright information in PMC

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