Review
doi: 10.1038/79598.
The question remains: is the spliceosome a ribozyme?
Affiliations
- PMID: 11017191
- DOI: 10.1038/79598
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Review
The question remains: is the spliceosome a ribozyme?
Nat Struct Biol.
2000 Oct.
Abstract
The two phosphoryl transfer steps of pre-mRNA splicing are catalyzed within the large ribonuclear protein machine called the spliceosome. The highly dynamic nature of the spliceosome has presented many challenges to a structural and mechanistic understanding of its catalytic core. While much evidence supports the popular hypothesis that the catalytic steps of pre-mRNA splicing are mediated by spliceosomal RNA, a role for protein in catalysis cannot yet be ruled out. A highly conserved protein, Prp8, is a component of the catalytic core. We review data consistent with the hypothesis that Prp8 functions as a cofactor to an RNA enzyme.
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