Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Oct;157(4):1055-62.
doi: 10.1016/S0002-9440(10)64619-6.

Prevalence of human papillomavirus DNA in different histological subtypes of cervical adenocarcinoma

E C Pirog  1 , B Kleter, S Olgac, P Bobkiewicz, J Lindeman, W G Quint, R M Richart, C Isacson
Affiliations

Prevalence of human papillomavirus DNA in different histological subtypes of cervical adenocarcinoma

E C Pirog et al. Am J Pathol. 2000 Oct.

Abstract

The prevalence of human papilloma virus (HPV) DNA in different histological subtypes of cervical adenocarcinoma and related tumors was examined using formalin-fixed, paraffin-embedded tissue samples from 105 primary cervical adenocarcinomas and adenosquamous carcinomas. Broad-spectrum HPV DNA amplification and genotyping was performed with the SPF10 primer set and line probe assay (LiPA), respectively. HPV DNA was detected in 82 of 90 (91%) mucinous adenocarcinomas, encompassing endocervical, intestinal, and endometrioid histological subtypes, and in nine of nine adenosquamous tumors (100%). HPV DNA was not detected in any nonmucinous adenocarcinomas including clear cell, serous, and mesonephric carcinomas (0/6). The most common viral types detected in adenocarcinoma were HPV 16 (50%) and HPV 18 (40%), followed by HPV 45 (10%), HPV52 (2%), and HPV 35 (1%). Multiple HPV types were detected in 9.7% of the cases. In conclusion, mucinous adenocarcinomas and adenosquamous carcinomas of the cervix demonstrate a very high prevalence of HPV DNA, similar to that reported for cervical squamous cell carcinoma. Only rare histological variants of cervical adenocarcinoma seem unrelated to HPV infection.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Identification of HPV genotypes using the LiPA. LiPA strips with hybridization bands indicating a single HPV type infection (lane 1, HPV 16; lane 2, HPV 18; lane 3, HPV 45; lane 4, HPV 31) and a multiple HPV type infection (lane 5, HPV 11 + 16; lane 6, HPV 16 + 18; lane 7, HPV 16 + 31; lane 8, HPV 16 + 39 + 66. Note: HPV 18 is reactive with two probes: 18 and c68.
See this image and copyright information in PMC

References

    1. Zheng T, Holford TR, Ma Z, Chen Y, Liu W, Ward BA, Boyle P: The continuing increase in adenocarcinoma of the uterine cervix: a birth cohort phenomenon. Int J Epidemiol 1996, 25:252-258 - PubMed
    1. Bosch FX, Manos MM, Munoz N, Sherman M, Jansen AM, Peto J, Schiffman MH, Moreno V, Kurman R, Shah KV: Prevalence of human papillomavirus in cervical cancer: a worldwide perspective. International Biological Study on Cervical cancer (IBSCC) Study Group. J Natl Cancer Inst 1995, 87:796-802 - PubMed
    1. van Muyden RC, ter Harmsel BW, Smedts FM, Hermans J, Kuijpers JC, Raikhlin NT, Petrov S, Lebedev A, Ramaekers FC, Trimbos JB, Kleter B, Quint WG: Detection and typing of human papillomavirus in cervical carcinomas in Russian women: a prognostic study. Cancer 1999, 85:2011-2016 - PubMed
    1. Skyldberg BM, Murray E, Lambkin H, Kelehan P, Auer GU: Adenocarcinoma of the uterine cervix in Ireland and Sweden: human papillomavirus infection and biologic alterations. Mod Pathol 1999, 12:675-682 - PubMed
    1. Ferguson AW, Svoboda-Newman SM, Frank TS: Analysis of human papillomavirus infection and molecular alterations in adenocarcinoma of the cervix. Mod Pathol 1998, 11:11-18 - PubMed