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. 2000 Oct;79(4):2150-4.
doi: 10.1016/S0006-3495(00)76462-9.

The unfolding/denaturation of immunogammaglobulin of isotype 2b and its F(ab) and F(c) fragments

A W Vermeer  1 , W Norde, A van Amerongen
Affiliations

The unfolding/denaturation of immunogammaglobulin of isotype 2b and its F(ab) and F(c) fragments

A W Vermeer et al. Biophys J. 2000 Oct.

Abstract

The unfolding and further denaturation of IgG and its F(ab) and F(c) fragments were studied both on a macroscopic and molecular level, using differential scanning calorimetry and circular dichroism spectroscopy, respectively. It was shown that the structural integrity of the F(ab) and F(c) units was retained after fragmentation of the IgG. The F(ab) fragment denatured at approximately 61 degrees C and the F(c) fragment at 71 degrees C. The structural transitions observed in the whole IgG is the sum effect of those determined for the isolated F(ab) and F(c) fragments.

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