doi: 10.1073/pnas.97.21.11135.
Sticking together
Affiliations
- PMID: 11027319
- PMCID: PMC34046
- DOI: 10.1073/pnas.97.21.11135
Item in Clipboard
Sticking together
Proc Natl Acad Sci U S A.
.
No abstract available
Figures
Gephyrin (93 kDa) binds glycine receptors and tubulin in
vitro and is required for clustering of glycine and
GABAA receptors at synapses; yet it does not associate with
GABAA receptors in vitro. GABARAP (14 kDa)
binds a subunit of the GABAA receptor, gephyrin, and
tubulin in vitro. Furthermore, GABARAP recruits
GABAA receptors into clusters at the plasma membrane in
heterologous quail fibroblast cells. However, GABARAP does not
colocalize with gephyrin or GABAA receptors in primary
neurons. Instead it is associated with intracellular vesicles. The data
suggest that GABARAP may mediate transport or targeting of gephyrin and
GABAA receptors, but another unknown protein may link
gephyrin and the GABAA receptor at synapses. Chen et
al. (12) also show that clustered receptors recruited to the
membrane by GABARAP in quail cells have a 4-fold reduced affinity for
GABA. If the GABARAP promoted clustering resembles that at synapses,
the implication is that clustering of GABAA receptors and
association with the subcellular cytoskeleton can dramatically alter
their kinetic properties.
Comment on
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The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse.Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8594-9. doi: 10.1073/pnas.97.15.8594. Proc Natl Acad Sci U S A. 2000. PMID: 10900017 Free PMC article.
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The gamma-aminobutyric acid type A (GABAA) receptor-associated protein (GABARAP) promotes GABAA receptor clustering and modulates the channel kinetics.Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11557-62. doi: 10.1073/pnas.190133497. Proc Natl Acad Sci U S A. 2000. PMID: 10984509 Free PMC article.
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