Direct selection of mutants restricting efficiency of suppression and misreading levels in E. coli B
- PMID: 1102920
- DOI: 10.1007/BF00703254
Direct selection of mutants restricting efficiency of suppression and misreading levels in E. coli B
Abstract
We describe a method for the direct selection of E. coli mutants restricting efficiency of suppression and misreading levels using a T4-coded nonsense suppressor. One mutant isolated has the phenotype expected for a restrictive mutant and may be ribosomal. Other possibilities are discussed.
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