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. 2000 Nov;182(21):6228-32.
doi: 10.1128/JB.182.21.6228-6232.2000.

Vancomycin resistance is associated with serine-containing peptidoglycan in Enterococcus gallinarum

P Grohs  1 , L Gutmann, R Legrand, B Schoot, J L Mainardi
Affiliations

Vancomycin resistance is associated with serine-containing peptidoglycan in Enterococcus gallinarum

P Grohs et al. J Bacteriol. 2000 Nov.

Abstract

In Enterococcus gallinarum SC1, a low-level vancomycin-resistant strain, only monomeric muropentapeptides with a C-terminal D-alanine were detected after growth without vancomycin. In contrast, in SC1 induced by vancomycin, as well as in AIB39, a constitutive vancomycin-resistant strain, monomeric and dimeric muropentapeptides with a C-terminal D-serine were detected.

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Figures

FIG. 1
FIG. 1
Separation of E. gallinarum cell wall muropeptides by reverse-phase HPLC. (A) Inducible strain SC1 not induced by vancomycin; (B) inducible strain SC1 induced by vancomycin. The arrows in panel A indicate the presence of pentapeptide/Ala; the arrows in panel B indicate the presence of pentapeptide/Ser.
FIG. 2
FIG. 2
Schematic representation of the MS-MS daughter spectrum of DS-D-penta/serine with an [M+H]+ ion at m/z 1,099.5.
See this image and copyright information in PMC

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