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. 2000 Jul 31;100(1-2):151-6.
doi: 10.1016/s0165-0270(00)00243-0.

A highly sensitive and selective radioimmunoassay for the measurement of neurotensin

P E Holtom  1 , C Wasuntarawat, S H Moss, S Aspley, P L Needham, G W Bennett
Affiliations

A highly sensitive and selective radioimmunoassay for the measurement of neurotensin

P E Holtom et al. J Neurosci Methods. .

Abstract

A highly selective and sensitive radioimmunoassay (RIA) for the detection of endogenous neurotensin (NT) has been developed. We have raised a C-terminally-directed antibody (CAb) that specifically binds 'biologically active' NT (NT and NT(8-13)) and that does not significantly cross-react with inactive NT metabolites or other bioactive peptides in the CNS. By reducing the volume of the assay to a low volume-RIA (30 microl), such that in vivo measurements can be made, we have increased the sensitivity (<0.3 fmol per tube), with inter- and intra-assay variations of 11.2 and 5.8%, respectively. Comparisons with similar methods of detecting NT have demonstrated that this RIA has a higher sensitivity than previously used RIA's and ELISA's. The data presented suggests that this sensitive RIA is a reliable method ideal for the detection of small quantities of biologically active NT.

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