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. 1975 Nov;12(5):1116-20.
doi: 10.1128/iai.12.5.1116-1120.1975.

Retention of motility and virulence of Treponema pallidum (Nichols strain) in vitro

S R Graves, P L Sandok, H M Jenkin, R C Johnson

Retention of motility and virulence of Treponema pallidum (Nichols strain) in vitro

S R Graves et al. Infect Immun. 1975 Nov.

Abstract

A maintenance medium for Treponema pallidum was designed to hold its Eh at the optimum for that organism, -10 to -110 mV. After 100% motile (freshly harvested) T. pallidum was inoculated into the medium, the motility of the treponemes decreased to 80% after 2 days, 50% after 3.5 days, and 0% after 9 days during incubation at 34 C. Full virulence was retained for 2 days, but it dropped rapidly thereafter, and the treponemes became avirulent by day 5.

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