Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Nov;88(2):205-215.
doi: 10.1016/S0304-3959(00)00353-5.

Cloning and functional expression of a human orthologue of rat vanilloid receptor-1

Philip Hayes  1 , Helen J Meadows, Martin J Gunthorpe, Mark H Harries, Malcolm D Duckworth, William Cairns, David C Harrison, Catherine E Clarke, Kathryn Ellington, Rab K Prinjha, Amanda J L Barton, Andrew D Medhurst, Graham D Smith, Simon Topp, Paul Murdock, Gareth J Sanger, John Terrett, Owen Jenkins, Christopher D Benham, Andrew D Randall, Isro S Gloger, John B Davis
Affiliations

Cloning and functional expression of a human orthologue of rat vanilloid receptor-1

Philip Hayes et al. Pain. 2000 Nov.

Abstract

Capsaicin, resiniferatoxin, protons or heat have been shown to activate an ion channel, termed the rat vanilloid receptor-1 (rVR1), originally isolated by expression cloning for a capsaicin sensitive phenotype. Here we describe the cloning of a human vanilloid receptor-1 (hVR1) cDNA containing a 2517 bp open reading frame that encodes a protein with 92% homology to the rat vanilloid receptor-1. Oocytes or mammalian cells expressing this cDNA respond to capsaicin, pH and temperature by generating inward membrane currents. Mammalian cells transfected with human VR1 respond to capsaicin with an increase in intracellular calcium. The human VR1 has a chromosomal location of 17p13 and is expressed in human dorsal root ganglia and also at low levels throughout a wide range of CNS and peripheral tissues. Together the sequence homology, similar expression profile and functional properties confirm that the cloned cDNA represents the human orthologue of rat VR1.

PubMed Disclaimer

References

    1. Bairoch A, Bucher P, Hofmann K. The PROSITE database, its status in 1997. Nucleic Acids Res. 1997;25:217-221.
    1. Baumann TK, Burchiel KJ, Ingram SL, Martenson ME. Responses of adult human dorsal root ganglion neurons in culture to capsaicin and low pH. Pain. 1996;65:31-38.
    1. Bevan S, Szolcsányi J. Sensory neuron-specific actions of capsaicin: mechanisms and applications. Trends Pharmacol Sci. 1990;11:330-333.
    1. Bevan S, Hothi S, Hughes G, James IF, Rang HP, Shah K, Walpole CSJ, Yeats JC. Capsazepine: a competitive antagonist of the sensory neurone excitant capsaicin. Br J Pharmacol. 1992;107:544-552.
    1. Bíró T, Brodie C, Modarres S, Lewin NE, Ács P, Blumberg PM. Specific vanilloid responses in C6 rat glioma cells. Mol Brain Res. 1998a;56:89-98.

MeSH terms