Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Sep 29;103(1):127-40.
doi: 10.1016/s0092-8674(00)00093-3.

Nucleophosmin/B23 is a target of CDK2/cyclin E in centrosome duplication

M Okuda  1 , H F Horn, P Tarapore, Y Tokuyama, A G Smulian, P K Chan, E S Knudsen, I A Hofmann, J D Snyder, K E Bove, K Fukasawa
Affiliations
Free article

Nucleophosmin/B23 is a target of CDK2/cyclin E in centrosome duplication

M Okuda et al. Cell. .
Free article

Abstract

In animal cells, duplication of centrosomes and DNA is coordinated. Since CDK2/cyclin E triggers initiation of both events, activation of CDK2/cyclin E is thought to link these two events. We identified nucleophosmin (NPM/B23) as a substrate of CDK2/cyclin E in centrosome duplication. NPM/B23 associates specifically with unduplicated centrosomes, and NPM/B23 dissociates from centrosomes by CDK2/cyclin E-mediated phosphorylation. An anti-NPM/B23 antibody, which blocks this phosphorylation, suppresses the initiation of centrosome duplication in vivo. Moreover, expression of a nonphosphorylatable mutant NPM/ B23 in cells effectively blocks centrosome duplication. Thus, NPM/B23 is a target of CDK2/cyclin E in the initiation of centrosome duplication.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources