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. 2000 Nov;38(11):4201-7.
doi: 10.1128/JCM.38.11.4201-4207.2000.

Application of tRNA intergenic spacer PCR for identification of Enterococcus species

M Baele  1 , P Baele, M Vaneechoutte, V Storms, P Butaye, L A Devriese, G Verschraegen, M Gillis, F Haesebrouck
Affiliations

Application of tRNA intergenic spacer PCR for identification of Enterococcus species

M Baele et al. J Clin Microbiol. 2000 Nov.

Abstract

tRNA intergenic spacer PCR (tDNA-PCR) was evaluated for its usefulness in the differentiation of enterococcal species of human and animal origin. This technique was carried out for 124 strains belonging to 17 enterococcal species and generated DNA fragments, which were separated by capillary electrophoresis. tDNA-PCR enabled us to discriminate for all species tested. Enterococcus faecium showed minor but reproducible differences with Enterococcus durans, while Enterococcus hirae was easily distinguishable. Enterococcus avium, Enterococcus malodoratus, and Enterococcus raffinosus generated highly similar though distinctive patterns.

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Figures

FIG. 1
FIG. 1
tDNA-PCR fingerprint patterns of enterococcal strains. a, E. faecalis 266/5371; b, E. faecium LMG 11423; c, E. hirae LMG 6399T; d, E. durans LMG 13604; e, E. avium LMG 107444T; f, E. malodoratus LMG 12300; g, E. raffinosus LMG 12888T; h, E. raffinosus LMG 14595; i, E. gallinarum LMG 16204; j, E. casseliflavus LMG 10745; k, E. flavescens LMG 13518; l, E. cecorum LMG 11744; m, E. columbae LMG 11740T. The x axis represents the fragment length in base pairs; the y axis represents the peak intensity.
FIG. 2
FIG. 2
Dendrogram obtained from tDNA-PCR fingerprints after similarity calculation with the in-house software and clustering with UPGMA using Neighbor software. Bar, distance of 10%.
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