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. 2000 Nov;38(11):4256-9.
doi: 10.1128/JCM.38.11.4256-4259.2000.

Rapid-cycle PCR for detection and typing of Mycoplasma pneumoniae in clinical specimens

F Kong  1 , S Gordon, G L Gilbert
Affiliations

Rapid-cycle PCR for detection and typing of Mycoplasma pneumoniae in clinical specimens

F Kong et al. J Clin Microbiol. 2000 Nov.

Abstract

We designed several new primers and modified previously described species- and type-specific primers targeting the Mycoplasma pneumoniae P1 adhesin gene. Optimized thermal profiles allowed one-step or nested PCR to be completed in less than 1 h. In 10 patients with pneumonia, M. pneumoniae type 1 was identified in 3 and type 2 in 7.

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FIG. 1
FIG. 1
Algorithm for detection, typing, and sequencing of M. pneumoniae using nested PCR.
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