Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Nov;38(11):4260-1.
doi: 10.1128/JCM.38.11.4260-4261.2000.

Evaluation of a commercial DNA enzyme immunoassay for detection of enterovirus reverse transcription-PCR products amplified from cerebrospinal fluid specimens

P P Young  1 , R S Buller, G A Storch
Affiliations

Evaluation of a commercial DNA enzyme immunoassay for detection of enterovirus reverse transcription-PCR products amplified from cerebrospinal fluid specimens

P P Young et al. J Clin Microbiol. 2000 Nov.

Abstract

We evaluated the DiaSorin DNA enzyme immunoassay (DEIA) kit for detection of enteroviral reverse transcription-PCR (RT-PCR) products amplified from cerebrospinal fluid. By use of an optical density of 0.05 as the absorbance cutoff, 35% of 198 specimens were PCR positive, whereas 16% were culture positive. DEIA was rapid and sensitive and can help implement enterovirus RT-PCR in clinical laboratories.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Ahmed A, Brito F, Goto C, Hickey S M, Olsen K D, Trujillo M, McCracken G H., Jr Clinical utility of the polymerase chain reaction for diagnosis of enteroviral meningitis in infancy. J Pediatr. 1996;131:393–397. - PubMed
    1. Hyypia T, Stalhandske P, Vainionpaa R, Pettersson U. Detection of enteroviruses by spot hybridization. J Clin Microbiol. 1984;19:436–438. - PMC - PubMed
    1. Muir P, Nicholson F, Jhetam M, Neogi S, Banatvala J E. Rapid diagnosis of enterovirus infection by magnetic bead extraction and polymerase chain reaction detection of enterovirus RNA in clinical specimens. J Clin Microbiol. 1993;31:31–38. - PMC - PubMed
    1. Muir P, Ras A, Klapper P E, Cleator G M, Korn K, Aepinus C, Fomsgaard A, Palmer P, Samuelsson A, Tenorio A, Weissbrich B, Van Loon A M. Multicenter quality assessment of PCR methods for detection of enteroviruses. J Clin Microbiol. 1999;37:1409–1414. - PMC - PubMed
    1. Pozo F, Casas I, Antonio T, Trallero G, Echevarria J M. Evaluation of a commercially available reverse transcription-PCR assay for a diagnosis of enteroviral infection in archival and prospectively collected cerebrospinal fluid specimens. J Clin Microbiol. 1998;36:1741–1745. - PMC - PubMed

Publication types

LinkOut - more resources