Quantitative gram stain interpretation criteria used by microbiology laboratories in Alberta, Canada
- PMID: 11060107
- PMCID: PMC87580
- DOI: 10.1128/JCM.38.11.4266-4268.2000
Quantitative gram stain interpretation criteria used by microbiology laboratories in Alberta, Canada
Abstract
Microbiology laboratories in Alberta, Canada, were surveyed to determine the quantitative interpretive criteria used to routinely read and report Gram stains. There was a wide variability in the quantitative reporting criteria cited for both cells and bacteria, with only 11 of 32 (34.4%) laboratories surveyed using the criteria recommended by the external proficiency-testing program. Lack of standardized criteria not only poses a problem in the grading of proficiency testing results but may also impact the quality of patient care.
Similar articles
-
Mechanisms to assess Gram stain interpretation proficiency of technologists at satellite laboratories.J Clin Microbiol. 2007 Nov;45(11):3754-8. doi: 10.1128/JCM.01632-07. Epub 2007 Aug 29. J Clin Microbiol. 2007. PMID: 17728479 Free PMC article.
-
Improving Gram stain proficiency in hospital and satellite laboratories that do not have microbiology.Clin Chem Lab Med. 2017 Mar 1;55(3):458-461. doi: 10.1515/cclm-2016-0556. Clin Chem Lab Med. 2017. PMID: 27658158
-
Positive blood cultures: can we always trust the Gram stain?Am J Clin Pathol. 2006 Nov;126(5):671-2. doi: 10.1309/A3A7-34C9-1JHP-HWB6. Am J Clin Pathol. 2006. PMID: 17050062 No abstract available.
-
[Identification of bacteria].Nihon Rinsho. 2007 Feb 28;65 Suppl 2 Pt. 1:163-8. Nihon Rinsho. 2007. PMID: 17455611 Review. Japanese. No abstract available.
-
Use of the gram stain in microbiology.Biotech Histochem. 2001 May;76(3):111-8. Biotech Histochem. 2001. PMID: 11475313 Review.
Cited by
-
Mechanisms to assess Gram stain interpretation proficiency of technologists at satellite laboratories.J Clin Microbiol. 2007 Nov;45(11):3754-8. doi: 10.1128/JCM.01632-07. Epub 2007 Aug 29. J Clin Microbiol. 2007. PMID: 17728479 Free PMC article.
-
Identification of causative agents of infective endocarditis by metagenomic next-generation sequencing of resected valves.Front Cell Infect Microbiol. 2025 Mar 13;15:1532257. doi: 10.3389/fcimb.2025.1532257. eCollection 2025. Front Cell Infect Microbiol. 2025. PMID: 40182766 Free PMC article.
-
Risk Model of Bacterial Coinfection in Children with Severe Viral Bronchiolitis.J Pediatr Intensive Care. 2017 Jun;6(2):103-108. doi: 10.1055/s-0036-1584810. Epub 2016 Jun 29. J Pediatr Intensive Care. 2017. PMID: 31073432 Free PMC article.
-
Wound swab quality grading is dependent on Gram smear screening approach.Sci Rep. 2023 Feb 23;13(1):3160. doi: 10.1038/s41598-023-29832-1. Sci Rep. 2023. PMID: 36823255 Free PMC article.
-
Biochemical but not compositional recovery of skin mucosal microbiome communities after disruption.Infect Drug Resist. 2019 Feb 13;12:399-416. doi: 10.2147/IDR.S185992. eCollection 2019. Infect Drug Resist. 2019. PMID: 30863123 Free PMC article.
References
-
- Bartlett J G, Ryan K J, Smith T F, Wilson W R. Cumitech 7A. 1987. Laboratory diagnosis of lower respiratory tract infections. Coordinating ed., J. A. Washington II. American Society for Microbiology, Washington, D.C.
-
- Bottone E J. The gram stain: the century-old quintessential rapid diagnostic test. Lab Med. 1988;19:288–291.
-
- Chapin K C, Murray P R. Strains. In: Murray P R, Baron E J, Pfaller M A, Tenover F C, Yolken R H, editors. Manual of clinical microbiology. 7th ed. Washington, D.C.: American Society for Microbiology; 1999. pp. 1674–1686.
-
- Chuard C, Antley C M, Reller L B. Clinical utility of cardiac valve Gram stain and culture in patients undergoing native valve replacement. Arch Pathol Lab Med. 1998;122:412–415. - PubMed
-
- Church D L, Don-Jo C, Unger B. Effects of restructuring on the performance of microbiology laboratories in Alberta. Arch Pathol Lab Med. 2000;124:357–361. - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources