Serodiagnosis of Bartonella bacilliformis infection by indirect fluorescence antibody assay: test development and application to a population in an area of bartonellosis endemicity
- PMID: 11060108
- PMCID: PMC87581
- DOI: 10.1128/JCM.38.11.4269-4271.2000
Serodiagnosis of Bartonella bacilliformis infection by indirect fluorescence antibody assay: test development and application to a population in an area of bartonellosis endemicity
Abstract
Bartonella bacilliformis causes bartonellosis, a potentially life-threatening emerging infectious disease seen in the Andes Mountains of South America. There are no generally accepted serologic tests to confirm the disease. We developed an indirect fluorescence antibody (IFA) test for the detection of antibodies to B. bacilliformis and then tested its performance as an aid in the diagnosis of acute bartonellosis. The IFA is 82% sensitive in detecting B. bacilliformis antibodies in acute-phase blood samples of laboratory-confirmed bartonellosis patients. When used to examine convalescent-phase sera, the IFA is positive in 93% of bartonellosis cases. The positive predictive value of the test is 89% in an area of Peru where B. bacilliformis is endemic and where the point prevalence of infection is 45%.
Figures
Similar articles
-
Antibodies to Bartonella bacilliformis as determined by fluorescence antibody test, indirect haemagglutination and ELISA.Trop Med Parasitol. 1985 Dec;36(4):183-5. Trop Med Parasitol. 1985. PMID: 3911360
-
Production of recombinant protein Pap31 and its application for the diagnosis of Bartonella bacilliformis infection.Ann N Y Acad Sci. 2005 Dec;1063:280-5. doi: 10.1196/annals.1355.045. Ann N Y Acad Sci. 2005. PMID: 16481528
-
Molecular detection and clinical characteristics of Bartonella bacilliformis, Leptospira spp., and Rickettsia spp. in the Southeastern Peruvian Amazon basin.BMC Infect Dis. 2018 Dec 4;18(1):618. doi: 10.1186/s12879-018-3541-7. BMC Infect Dis. 2018. PMID: 30514235 Free PMC article.
-
Laboratory diagnosis of Bartonella infections.Clin Lab Med. 2002 Dec;22(4):937-62. doi: 10.1016/s0272-2712(02)00017-3. Clin Lab Med. 2002. PMID: 12489289 Review.
-
[Bartonella and bartonellosis--emerging and re-emerging infections: epidemiology, clinical course, diagnosis].Zh Mikrobiol Epidemiol Immunobiol. 2005 Jan-Feb;(1):83-9. Zh Mikrobiol Epidemiol Immunobiol. 2005. PMID: 15773411 Review. Russian.
Cited by
-
Whole-Genome Analysis of Bartonella ancashensis, a Novel Pathogen Causing Verruga Peruana, Rural Ancash Region, Peru.Emerg Infect Dis. 2017 Mar;23(3):430-438. doi: 10.3201/eid2303.161476. Emerg Infect Dis. 2017. PMID: 28221130 Free PMC article.
-
Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples.PLoS Negl Trop Dis. 2016 Mar 9;10(3):e0004529. doi: 10.1371/journal.pntd.0004529. eCollection 2016 Mar. PLoS Negl Trop Dis. 2016. PMID: 26959642 Free PMC article.
-
Complete Genome Sequence of Bartonella ancashensis Strain 20.00, Isolated from the Blood of a Patient with Verruga Peruana.Genome Announc. 2015 Nov 5;3(6):e01217-15. doi: 10.1128/genomeA.01217-15. Genome Announc. 2015. PMID: 26543106 Free PMC article.
-
Population genetic analysis of Bartonella bacilliformis isolates from areas of peru where Carrion's disease is endemic and epidemic.J Clin Microbiol. 2004 Aug;42(8):3675-80. doi: 10.1128/JCM.42.8.3675-3680.2004. J Clin Microbiol. 2004. PMID: 15297516 Free PMC article.
-
Immunogenic Peptides from Pap31 and SCS-α of Bartonella bacilliformis: One Step Closer to a Rapid Diagnostic Tool for Carrion's Disease.Pathogens. 2021 Jul 21;10(8):917. doi: 10.3390/pathogens10080917. Pathogens. 2021. PMID: 34451381 Free PMC article.
References
-
- Amano Y, Rumbea J, Knobloch J, Olson J, Kron M. Bartonellosis in Ecuador: serosurvey and current status of cutaneous verrucous disease. Am J Trop Med Hyg. 1997;57:174–179. - PubMed
-
- Birtles R J, Canales J, Ventosilla P, Alvarez E, Guerra H, Llanos-Cuentas A, Raoult D, Doshi N, Harrison T G. Survey of Bartonella species infecting intradomicillary animals in the Huayllacallan Valley, Ancash, Peru, a region endemic for human bartonellosis. Am J Trop Med Hyg. 1999;60:799–805. - PubMed
-
- Caceres-Rios H, Rodriguez-Tafur J, Bravo-Puccio F, Maguina-Vargas C, Diaz C S, Ramos D C, Patarca R. Verruga peruana: an infectious endemic angiomatosis. Crit Rev Oncog. 1995;6:47–56. - PubMed
-
- Ellis B, Regnery R, Beati L, Bacella F, Rood M, Glass G, Marston E, Ksiazek T, Jones D, Childs J. Rats of the genus Rattus are reservoir hosts for pathogenic Bartonella species: an old world origin for a new world disease? J Infect Dis. 1999;180:220–224. - PubMed
-
- Ellis B, Rotz L, Leake J, Samalvides F, Bernable J, Ventura G, Padilla C, Villaseca P, Beati L, Regnery R, Childs J, Olson J, Carrillo C. An outbreak of acute bartonellosis (Oroya fever) in the Urubamba region of Peru, 1998. Am J Trop Med Hyg. 1999;61:344–349. - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases