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. 2000 Nov;7(11):1013-7.
doi: 10.1038/80917.

Structural basis for the topological specificity function of MinE

G F King  1 , Y L Shih, M W Maciejewski, N P Bains, B Pan, S L Rowland, G P Mullen, L I Rothfield
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Structural basis for the topological specificity function of MinE

G F King et al. Nat Struct Biol. 2000 Nov.

Abstract

Correct positioning of the division septum in Escherichia coli depends on the coordinated action of the MinC, MinD and MinE proteins. Topological specificity is conferred on the MinCD division inhibitor by MinE, which counters MinCD activity only in the vicinity of the preferred midcell division site. Here we report the structure of the homodimeric topological specificity domain of Escherichia coli MinE and show that it forms a novel alphabeta sandwich. Structure-directed mutagenesis of conserved surface residues has enabled us to identify a spatially restricted site on the surface of the protein that is critical for the topological specificity function of MinE.

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  • How does a bacterium find its middle?
    RayChaudhuri D, Gordon GS, Wright A. RayChaudhuri D, et al. Nat Struct Biol. 2000 Nov;7(11):997-9. doi: 10.1038/81014. Nat Struct Biol. 2000. PMID: 11062548 No abstract available.

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