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. 2000 Nov;44(5):799-802.
doi: 10.1002/1522-2594(200011)44:5<799::aid-mrm18>3.0.co;2-s.

Determination of pH using water protons and chemical exchange dependent saturation transfer (CEST)

K M Ward  1 , R S Balaban
Affiliations

Determination of pH using water protons and chemical exchange dependent saturation transfer (CEST)

K M Ward et al. Magn Reson Med. 2000 Nov.

Abstract

Solution pH was measured using water proton NMR via chemical exchange dependent saturation transfer (CEST) with selected chemical exchange sites. Several useful pH-sensitive proton chemical exchange agents were found: 5,6-dihydrouracil, 5-hydroxytryptophan, and a combination of 5-hydroxytryptophan and 2-imidazolidinethione. A ratiometric approach was developed that permitted pH determinations that were independent of water T(1) or exchange site concentration.

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