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. 1975 Mar-Apr;40(2):389-94.

[Isolation and characteristics of homopolyribonucleotide synthesized in toluene-treated E. coli cells]

[Article in Russian]
S P Mardashchev
  • PMID: 1106777

[Isolation and characteristics of homopolyribonucleotide synthesized in toluene-treated E. coli cells]

[Article in Russian]
S P Mardashchev. Biokhimiia. 1975 Mar-Apr.

Abstract

Optimal conditions of homopolyribonucleotide (poly-A-14C) synthesis in toluene-treated E. coli cells under incubation with ADP-14C, Mg2+ and tris. HCl buffer (pH 8.0) are studied. Optimal Mg2+ concentration was 0.75.-10(-3) M. Heterogeneity of the isolated poly-A-14C from E. coli cell was demonstrated by means of sucrose density gradient (5-20%) centrifugation and polyacrylamide gel electrophoresis. Actinomycin D was found not to affect the reaction rate of polymerization of ADP-14C, UDP-14C and GDP-14C, catalyzed by polynucleotide phosphorylase in toluene-treated E. coli cells.

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