Biosynthesis of a specific lipoprotein of the Escherichia coli outer membrane on polyribosomes

Abstract

With use of polyribosomes isolated from Escherichia coli, biosynthesis of a specific lipoprotein of the E. coli outer membrane was investigated. The products of the cell-free system were analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis and immunoprecipitation with anti-lipoportein serum, followed by sodium dodecylsulfate-polyacrylamide gel electrophoresis. It was found that one of the major products was the lipoprotein of the E. coli outer membrane. The lipoprotein was also found to be preferentially synthsized on smaller polyribosomes.