Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Nov 24;141(2):163-71.
doi: 10.1007/BF00267681.

Genetic consequences of transfection with heterduplex bacteriophage lambda DNA

R L White, M S Fox

Genetic consequences of transfection with heterduplex bacteriophage lambda DNA

R L White et al. Mol Gen Genet. .

Abstract

The role of rectification of heteroduplex heterozygotes in the formation of recombinant genotypes involving closely linked markers has been examined. Heteroduplex molecules of bacteriophage lambda DNA, heterozygous at several alleles, have been constructed and the genetic composition of phage present in infective centers derived by transfection with such molecules has been determined. Allele loss and concomitant recombinant formation is frequent, and appears to reflect marker specificity as well as specificities imposed by whether or not the transfection recipient is permissive or nonpermissive for DNA duplication of the transfecting genome. The observations support the proposal that many, perhaps most, of the events involving separation of closely linked markers occur by rectification of non-recombinant heterozygotes.

PubMed Disclaimer

References

    1. J Gen Physiol. 1966 Jul;49(6):183-96 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2202-6 - PubMed
    1. Virology. 1962 May;17:44-55 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1544-8 - PubMed
    1. J Mol Biol. 1964 Apr;8:516-40 - PubMed

LinkOut - more resources