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. 1975 Dec;72(12):4780-4.
doi: 10.1073/pnas.72.12.4780.

Content of elongation factor Tu in Escherichia coli

A V Furano

Content of elongation factor Tu in Escherichia coli

A V Furano. Proc Natl Acad Sci U S A. 1975 Dec.

Abstract

The content of elongation factor Tu in E. coli B has been determined both by radioimmune assay and by GDP binding. The two assays gave comparable results: cells growing at 2 doublings per hour contained about 8 molecules of Tu per ribosome, whereas those growing at 0.22 doublings per hour contained about 14 molecules per ribosome. These levels resemble those reported for tRNA, in contrast with the 1:1 ratio of factor to ribosomes reported for elongation factors Ts and G.

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