Electron microscopic observations on the meiotic karyotype of diploid and tetraploid Saccharomyces cerevisiae

Abstract

Certain strains of Saccharomyces cerevisiae contain visible segments of synaptonemal complex which are apparent components of bivalents in pachytene of meiotic prophase. The synaptonemal complex has the typical width in the frontal plane but is unusually thin in the sagittal plane, thus accounting for its poor visibility. Amorphous densities situated adjacent to the central element occur at intervals suggesting their coincidence with sites of crossing over. Reconstruction of the synaptonemal complex from serial sections has permitted karyotypic analysis. The number of segments of synaptonemal complex and the distribution of their legths is consistent with the genetic map. Two, possibly three, segments enter the nucleolus as if bearing sequences encoding ribosomal RNA. Reconstruction of tetraploid nuclei reveals an approximate doubling of the diploid chromosome number and confirms the pattern of nucleolar entry. Quadrivalent pairing is evident between the pairs of synaptonemal complex segments in the tetraploid nuclei.