Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Dec;72(12):5099-102.
doi: 10.1073/pnas.72.12.5099.

Immunosurgery of mouse blastocyst

D Solter, B B Knowles

Immunosurgery of mouse blastocyst

D Solter et al. Proc Natl Acad Sci U S A. 1975 Dec.

Abstract

Mouse blastocysts with and without zonae pellucidae are susceptible to complement-dependent antibody cytotoxicity. Exposure of blastocysts to rabbit anti-mouse serum together with complement results in the death of all cells; however, when blastocysts are exposed to antiserum alone and then transferred to guinea pig complement, only the trophoblastic cells are killed. These results suggest that the mouse blastocyst is not permeable for certain antibodies. The inner cell masses can easily be separated from the remnants of trophoblastic cells and are then able to grow and differentiate in vitro. This method of immunosurgery can be used to obtain large quantities of pure inner cell masses in a relatively short period of time.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Dev Biol. 1975 Jun;44(2):313-25 - PubMed
    1. J Reprod Fertil. 1975 Jan;42(1):153-7 - PubMed
    1. Anat Rec. 1974 Oct;180(2):263-79 - PubMed
    1. Exp Cell Res. 1974 Apr;85(2):424-8 - PubMed
    1. J Embryol Exp Morphol. 1973 Jun;29(3):601-15 - PubMed

Publication types

LinkOut - more resources