A precursor of cytoplasmic actin in cultured Drosophila cells
- PMID: 110818
- PMCID: PMC2110386
- DOI: 10.1083/jcb.81.3.680
A precursor of cytoplasmic actin in cultured Drosophila cells
Abstract
In continuous lines of cultured Drosophila cells two forms of cytoplasmic actin, designated II and III, are detected after a 25- to 120-min pulse label with [35S]methionine. However, only one of these, actin II, accumulates in the cell. With the use of a pulse-chase protocol and two-dimensional gel electrophoresis, it has been found that actin III is synthesized as a precursor of the more stable cytoplasmic actin II. The half-life of actin III was estimated to be approximately 50 min.
Similar articles
-
Myogenesis in primary cell cultures from Drosophila melanogaster: protein synthesis and actin heterogeneity during development.Cell. 1978 Apr;13(4):589-98. doi: 10.1016/0092-8674(78)90210-6. Cell. 1978. PMID: 418880
-
Multiple actins in Drosophila melanogaster.J Cell Biol. 1979 Jul;82(1):86-92. doi: 10.1083/jcb.82.1.86. J Cell Biol. 1979. PMID: 113417 Free PMC article.
-
Actin isoform synthesis by cultured cardiac myocytes. Effects of doxorubicin.Lab Invest. 1987 Mar;56(3):295-301. Lab Invest. 1987. PMID: 3821069
-
Determination of actin messenger RNA in cultures of differentiating embryonic chick skeletal muscle.Proc Natl Acad Sci U S A. 1974 Nov;71(11):4467-71. doi: 10.1073/pnas.71.11.4467. Proc Natl Acad Sci U S A. 1974. PMID: 4530996 Free PMC article.
-
Bulk cytoplasmic actin and its functions in meiosis and mitosis.Curr Biol. 2011 Oct 11;21(19):R825-30. doi: 10.1016/j.cub.2011.07.043. Curr Biol. 2011. PMID: 21996509 Review.
Cited by
-
Drosophila cells and ecdysterone: a model system for gene regulation.In Vitro. 1984 Dec;20(12):959-74. doi: 10.1007/BF02619669. In Vitro. 1984. PMID: 6442267 Review.
-
Actin acetylation in Drosophila tissue culture cells.Biochem Genet. 1981 Apr;19(3-4):321-31. doi: 10.1007/BF00504277. Biochem Genet. 1981. PMID: 6166297
-
cDNA clones for the ecdysone-inducible polypeptide (EIP) mRNAs of Drosophila Kc cells.EMBO J. 1984 Jan;3(1):235-43. doi: 10.1002/j.1460-2075.1984.tb01790.x. EMBO J. 1984. PMID: 16453496 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases