Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Dec;44(12):3447-50.
doi: 10.1128/AAC.44.12.3447-3450.2000.

Comparative In vitro activities of daptomycin and vancomycin against resistant gram-positive pathogens

D R Snydman  1 , N V Jacobus, L A McDermott, J R Lonks, J M Boyce
Affiliations

Comparative In vitro activities of daptomycin and vancomycin against resistant gram-positive pathogens

D R Snydman et al. Antimicrob Agents Chemother. 2000 Dec.

Abstract

The in vitro activity of daptomycin against 224 current gram-positive clinical isolates including vancomycin-resistant Enterococcus faecium (VREF), methicillin-resistant Staphylococcus aureus (MRSA), methicillin-resistant Staphylococcus spp. (MRSS), and penicillin-resistant Streptococcus pneumoniae (PRSP) was evaluated. The MICs at which 90% of isolates are inhibited for daptomycin and vancomycin, respectively, were as follows: MRSA, 1 and 2 microg/ml; MRSS, 1 and 4 microg/ml; PRSP, 1 and 0.5 microg/ml; and VREF, 2 and >64 microg/ml. Daptomycin was bactericidal against 82% of 17 VREF isolates. The antibacterial activity of daptomycin was strongly dependent on the calcium concentration of the medium. Daptomycin was active against all gram-positive cocci tested.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Effect of daptomycin concentration on in vitro time-kill kinetics for E. faecium SSL 110 (VRE) in MH II broth (25 μg of Ca2+/ml).
FIG. 2
FIG. 2
Effect of calcium concentration in the test medium (MH broth) on activity of daptomycin against S. aureus SSL 50 (MRSA). (A) Daptomycin concentration equivalent to 1× the MIC (1 μg/ml). (B) Daptomycin concentration equivalent to 4× the MIC (4 μg/ml).
See this image and copyright information in PMC

References

    1. Benson C A, Beaudette F, Trenholm G. Comparative in vitro activity of LY146032, a new peptide, with vancomycin and eight other agents against gram-positive organisms. J Antimicrob Chemother. 1987;20:191–196. - PubMed
    1. Eliopoulos G M, Willey S, Reiszner E, Spitzer P G, Caputo G, Moellering R C. In vitro and in vivo activity of LY146032, a new cyclic lipopeptide antibiotic. Antimicrob Agents Chemother. 1986;30:532–535. - PMC - PubMed
    1. Goering R V, Winters M A. Rapid method for epidemiological evaluation of gram-positive cocci by field inversion gel electrophoresis. J Clin Microbiol. 1992;30:557–580. - PMC - PubMed
    1. Hanberger H, Nilsson L E, Maller R, Isaksson B. Pharmacodynamics of daptomycin and vancomycin on E. faecalis and S. aureus demonstrated by studies of initial killing and postantibiotic effect and influence of Ca2+ and albumin on these drugs. Antimicrob Agents Chemother. 1991;35:1710–1716. - PMC - PubMed
    1. Lakey J H, Ptak M. Fluorescence indicates a calcium-dependent interaction between the lipopeptide antibiotic LY 146032 and phospholipid membranes. Biochemistry. 1988;27:4639–4645. - PubMed

Publication types

LinkOut - more resources