Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Dec;68(12):7149-51.
doi: 10.1128/IAI.68.12.7149-7151.2000.

Identification of genes present specifically in a virulent strain of Klebsiella pneumoniae

Y C Lai  1 , S L Yang, H L Peng, H Y Chang
Affiliations

Identification of genes present specifically in a virulent strain of Klebsiella pneumoniae

Y C Lai et al. Infect Immun. 2000 Dec.

Abstract

Klebsiella pneumoniae is a common cause of septicemia and urinary tract infections. The PCR-supported genomic subtractive hybridization was employed to identify genes specifically present in a virulent strain of K. pneumoniae. Analysis of 25 subtracted DNA clones has revealed 19 distinct nucleotide sequences. Two of the sequences were found to be the genes encoding the transposase of Tn3926 and a capsule polysaccharide exporting enzyme. Three sequences displayed moderate homology with bvgAS, which encodes a two-component signal transduction system in Bordetella pertussis. The rest of the sequences did not exhibit homology with any known genes. The distribution of these novel sequences varied greatly in K. pneumoniae clinical isolates, reflecting the heterogeneous nature of the K. pneumoniae population.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Distribution of bvgS-like gene in K. pneumoniae clinical isolates. Cell lysates of 95 different K. pneumoniae blood isolates were spotted on a nitrocellulose membrane, and the presence of the bvgS homolog was detected by using labeled AJ293852 DNA as a probe. A1, K. pneumoniae CG43; A2, M5a1; A3, E. coli XL-1Blue; A4 to I2, different K. pneumoniae clinical isolates; I3, purified AJ293852 DNA.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Abu Kwaik Y, Pederson L L. The use of differential display-PCR to isolate and characterize a Legionella pneumophila locus induced during the intracellular infection of macrophages. Mol Microbiol. 1996;21:543–556. - PubMed
    1. Akopyants N S, Fradkov A, Diatchenko L, Hill J E, Siebert P D, Lukyanov S A, Sverdlov E D, Berg D E. PCR-based subtractive hybridization and differences in gene content among strains of Helicobacter pylori. Proc Natl Acad Sci USA. 1998;95:13108–13113. - PMC - PubMed
    1. Altschul S F, Gish W, Miller W, Myers E W, Lipman D J. Basic local aligment search tools. J Mol Biol. 1990;215:403–410. - PubMed
    1. Arakawa Y, Wacharotayankun R, Nagatsuka T, Ito H, Kato N, Ohta M. Genomic organization of the Klebsiella pneumoniae cps region responsible for serotype K2 capsular polysaccharide synthesis in the virulent strain Chedid. J Bacteriol. 1995;177:1788–1796. - PMC - PubMed
    1. Aricó B, Scarlato V, Monack D M, Falkow S, Rappuoli R. Structural and genetic analysis of the bvg locus in Bordetella species. Mol Microbiol. 1991;5:2481–2492. - PubMed

Publication types

LinkOut - more resources