The action spectrum for maximal photosensitivity of germination

Abstract

Fruits of Garden Lettuce, imbibed in 0.01 M KNO3, were depleted of maternal active phytochrome B by saturating deep-red exposure and photosensitized by chilling for 1 week at 4 degrees C. Twenty saturated fluence-response curves for photoinduced germination were elaborated between 300 and 800 nm, using exposure periods from 6 to 600 s at 22.5 degrees C; there is linear and closely parallel regression in the logarithmic probability net. The reciprocals of the half-response fluences obtained gave the apparent conversion spectrum of the controlling pigments and this was corrected for the transmittance of the seed-coat. It is a phytochrome spectrum of Pr with photoconversion cross-sections of 1.2 x 10(9) and 4.5 x 10(3) m2 mol-1 at 666 and 800 nm, respectively. This means that for half-saturated germination of sensitized seed, fewer than 1 out of 200,000 phytochrome A molecules have to be photoconverted to Pfr, and no photo-reversibility by deep-red was found. Therefore, all spectral colours of nightly moon- or skylight should stimulate the germination of sensitized weed seeds if they are exposed at the soil surface between sequential tillage operations.