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Comparative Study
. 2000 Dec;66(12):5499-502.
doi: 10.1128/AEM.66.12.5499-5502.2000.

Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites

L Xiao  1 , J Limor, U M Morgan, I M Sulaiman, R C Thompson, A A Lal
Affiliations
Comparative Study

Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites

L Xiao et al. Appl Environ Microbiol. 2000 Dec.

Abstract

Nucleotide sequences of the Cryptosporidium oocyst wall protein (COWP) gene were obtained from various Cryptosporidium spp. (C. wrairi, C. felis, C. meleagridis, C. baileyi, C. andersoni, C. muris, and C. serpentis) and C. parvum genotypes (human, bovine, monkey, marsupial, ferret, mouse, pig, and dog). Significant diversity was observed among species and genotypes in the primer and target regions of a popular diagnostic PCR. These results provide useful information for COWP-based molecular differentiation of Cryptosporidium spp. and genotypes.

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Figures

FIG. 1
FIG. 1
Variation in the COWP nucleotide sequences among nine Cryptosporidium spp. and eight C. parvum genotypes in the region targeted by the PCR-RFLP diagnostic tool (11). Dots denote sequence identity to the bovine genotype of C. parvum. Dashes indicate sequence ubiquity. The monkey and human genotypes of C. parvum had identical sequence in this region.
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