Evaluation of R-Mix FreshCells in shell vials for detection of respiratory viruses
- PMID: 11101618
- PMCID: PMC87659
- DOI: 10.1128/JCM.38.12.4660-4662.2000
Evaluation of R-Mix FreshCells in shell vials for detection of respiratory viruses
Abstract
The performance of a mixture of mink lung and A549 cell lines in shell vials (MSVs) for the detection of respiratory viruses in 159 specimens was evaluated. MSVs, conventional culture, and direct immunofluorescence assay identified 96, 85, and 67% of the influenza A virus-positive specimens, respectively. MSVs provided both a high degree of sensitivity and rapid turnaround times for the detection of influenza A virus.
Similar articles
-
R-Mix cells are faster, at least as sensitive and marginally more costly than conventional cell lines for the detection of respiratory viruses.J Clin Virol. 2001 Aug;22(1):101-10. doi: 10.1016/s1386-6532(01)00171-8. J Clin Virol. 2001. PMID: 11418357
-
Enhanced detection of respiratory viruses using cryopreserved R-Mix ReadyCells.J Clin Virol. 2008 Jul;42(3):264-7. doi: 10.1016/j.jcv.2008.03.015. Epub 2008 May 7. J Clin Virol. 2008. PMID: 18467164
-
Rapid and sensitive detection of respiratory virus infections for directed antiviral treatment using R-Mix cultures.J Clin Virol. 2002 Feb;24(1-2):107-15. doi: 10.1016/s1386-6532(01)00239-6. J Clin Virol. 2002. PMID: 11744435
-
Prospective comparison of R-mix shell vial system with direct antigen tests and conventional cell culture for respiratory virus detection.J Clin Virol. 2007 Mar;38(3):210-6. doi: 10.1016/j.jcv.2006.12.015. Epub 2007 Jan 16. J Clin Virol. 2007. PMID: 17229589 Free PMC article.
-
[The rapid detection kit based on neuraminidase activity of influenza virus].Nihon Rinsho. 2000 Nov;58(11):2234-7. Nihon Rinsho. 2000. PMID: 11225310 Review. Japanese.
Cited by
-
Monoclonal antibodies versus reverse transcription-PCR for detection of respiratory viruses in a patient population with respiratory tract infections admitted to hospital.J Med Virol. 2005 Feb;75(2):336-47. doi: 10.1002/jmv.20276. J Med Virol. 2005. PMID: 15602736 Free PMC article.
-
Relevance of influenza a virus detection by PCR, shell vial assay, and tube cell culture to rapid reporting procedures.J Clin Microbiol. 2006 Sep;44(9):3366-7. doi: 10.1128/JCM.00314-06. J Clin Microbiol. 2006. PMID: 16954274 Free PMC article.
-
Sensitivity of respiratory virus culture when screening with R-mix fresh cells.J Clin Microbiol. 2004 Jan;42(1):79-82. doi: 10.1128/JCM.42.1.79-82.2004. J Clin Microbiol. 2004. PMID: 14715735 Free PMC article.
-
Multicenter Clinical Evaluation of the Alere i Respiratory Syncytial Virus Isothermal Nucleic Acid Amplification Assay.J Clin Microbiol. 2018 Feb 22;56(3):e01777-17. doi: 10.1128/JCM.01777-17. Print 2018 Mar. J Clin Microbiol. 2018. PMID: 29263208 Free PMC article.
-
Rapid diagnosis of influenza: state of the art.Clin Lab Med. 2014 Jun;34(2):365-85. doi: 10.1016/j.cll.2014.02.009. Clin Lab Med. 2014. PMID: 24856533 Free PMC article. Review.
References
MeSH terms
LinkOut - more resources
Full Text Sources