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. 2000 Dec 1;34(2):147-155.
doi: 10.1111/j.1574-6941.2000.tb00764.x.

Screening of sulfate-reducing bacteria in colonoscopy samples from healthy and colitic human gut mucosa

V Zinkevich V  1 , IB Beech
Affiliations
Free article

Screening of sulfate-reducing bacteria in colonoscopy samples from healthy and colitic human gut mucosa

V Zinkevich V et al. FEMS Microbiol Ecol. .
Free article

Abstract

A PCR-based approach combined with microbiological cultivation methods was employed to determine the occurrence of sulfate-reducing bacteria (SRB) in colon biopsy samples from ulcerative colitis patients and from non-colitic controls. The detection of mucosa-associated SRB was carried out by digoxigenin-dUTP-labelled PCR amplification, in liquid Postgate medium B and in a new liquid medium, termed VM medium I. Using Postgate medium B, the growth of SRB was confirmed in 92% of the colitic specimens and in 52% of non-colitic samples. However, PCR analysis and incubation in VM medium I detected SRB in 100% of biopsy material indicating ubiquitous presence of SRB in human colon mucosa.

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