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. 1975 Jan;29(1):17-20.
doi: 10.1128/am.29.1.17-20.1975.

Preparation of 14C-labeled sterigmatocystin in liquid media

D P Hsieh, S L Yang

Preparation of 14C-labeled sterigmatocystin in liquid media

D P Hsieh et al. Appl Microbiol. 1975 Jan.

Abstract

14C-labeled sterigmatocystin was prepared from surface cultures of Aspergillus versicolor A-18074 maintained in liquid media by multiple additions of (1-14C)acetate to the cultures. The highest yield of 7.75 mg/10 ml was found with a sucrose-asparagine-ammonium medium in which more than 3% of the radioactivity of the added (1-14C)acetate was recovered ithe purified (ring-14C) sterigmatocystin. The method offers an easy way to prepare 14C-labeled sterigmatocystin for studies of this mycotoxin.

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