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. 2000 Dec;10(12):1941-57.
doi: 10.1101/gr.gr1499r.

Single QTL effects, epistasis, and pleiotropy account for two-thirds of the phenotypic F(2) variance of growth and obesity in DU6i x DBA/2 mice

G A Brockmann  1 , J Kratzsch, C S Haley, U Renne, M Schwerin, S Karle
Affiliations

Single QTL effects, epistasis, and pleiotropy account for two-thirds of the phenotypic F(2) variance of growth and obesity in DU6i x DBA/2 mice

G A Brockmann et al. Genome Res. 2000 Dec.

Abstract

Genes influencing body weight and composition and serum concentrations of leptin, insulin, and insulin-like growth factor I (IGF-I) in nonfasting animals were mapped in an intercross of the extreme high-growth mouse line DU6i and the inbred line DBA/2. Significant loci with major effects (F > 7.07) for body weight, obesity, and muscle weight were found on chromosomes 1, 4, 5, 7, 11, 12, 13, and 17, for leptin on chromosome 14, for insulin on chromosome 4, and for IGF-I on chromosome 10 at the Igf1 gene locus itself and on chromosome 18. Significant interaction between different quantitative trait loci (QTL) positions was observed (P < 0.01). Evidence was found that loci having small direct effect on growth or obesity contribute to the obese phenotype by gene-gene interaction. The effects of QTLs, epistasis, and pleiotropy account for 64% and 63% of the phenotypic variance of body weight and fat accumulation and for over 32% of muscle weight and serum concentrations of leptin, and IGF-I in the F(2) population of DU6i x DBA/2 mice. [The quantitative trait loci described in this paper have been submitted to the Mouse Genome Database.]

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Figures

Figure 1
Figure 1
Depiction of chromosomes harboring QTLs newly identified in this study. QTL symbols of newly identified QTLs were labeled in bold text in the last column of Table 5. Shown are the chromosomal positions and confidence intervals of significant QTLs for body weight (Bw*), abdominal fat weight (Afw*), abdominal fat percentage (Afpq*), muscle weight (Mwq*), liver weight (Lwq*), kidney weight (Kwq*), spleen weight (Swq*), leptin (Lepq*), insulin (Insq*), and IGF-I (Igf1q*) relative to map positions of candidate genes for the QTL effects. Candidate genes were taken from the Mouse Genome Database (MGD), section endocrine defects, hormones, growth, and obesity (MGD), and from the Human Obesity Gene Map (Chagnon et al. 2000). The human homology is shown on the left side of every chromosome.
See this image and copyright information in PMC

References

    1. Brockmann G, Timtchenko D, Das P, Renne U, Freyer G, Kuhla S, Teuscher F, Wolf J, Kühn C, Schwerin M. Detection of QTL for body weight and body fat content in mice using genetic markers. J Anim Breed Genet. 1996;113:373–379.
    1. Brockmann GA, Haley CS, Renne U, Knott SA, Schwerin M. QTLs affecting body weight and fatness from a mouse line selected for high growth. Genetics. 1998;150:369–381. - PMC - PubMed
    1. Bünger L, Herrendörfer G, Renne U. Results of long term selection for growth traits in laboratory mice. Proceedings of the 4th World Congress on Genetics Applied to Livestock Production, Univ Edinburgh, Edinburgh, Scotland. 1990;13:321–324.
    1. Chagnon YC, Perusse L, Weisnagel SJ, Rankinen T, Bouchard C. The human obesity gene map: The 1999 update. Obes Res. 2000;8:89–117. - PubMed
    1. Cheverud JM, Routman EJ. Epistasis and its contribution to genetic variance components. Genetics. 1995;130:1455–1461. - PMC - PubMed

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