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. 1979 Jun;76(6):2964-8.
doi: 10.1073/pnas.76.6.2964.

Partial purification of Rho (D) antigen from Rh positive and negative erythrocytes

F V Plapp, M M Kowalski, L Tilzer, P J Brown, B J Evans, M Chiga

Partial purification of Rho (D) antigen from Rh positive and negative erythrocytes

F V Plapp et al. Proc Natl Acad Sci U S A. 1979 Jun.

Abstract

A rapid method is described for partial purification of Rho(D) antigen from sodium deoxycholate-solubilized erythrocyte membranes by affinity chromatography on a column coupled with anti-Rho(D) IgG. The Rho(D) antigen is a low molecular weight membrane protein that comigrates with the lipid zone of erythrocytes during sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Similar quantities of Rho(D) antigen were present in Rh positive and negative erythrocytes. However, in the latter erythrocytes the antigen may not be exposed to the external cell surface, explaining why these cells are not agglutinated by anti-Rho(D) antiserum. LW antigen was also present in the fraction containing Rho(D) antigen. The genetic implications of these findings are discussed.

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