Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001 Jan;67(1):459-61.
doi: 10.1128/AEM.67.1.459-461.2001.

Application of rapid dot blot immunoassay for detection of Salmonella enterica serovar enteritidis in eggs, poultry, and other foods

M A Yoshimasu  1 , J Zawistowski
Affiliations

Application of rapid dot blot immunoassay for detection of Salmonella enterica serovar enteritidis in eggs, poultry, and other foods

M A Yoshimasu et al. Appl Environ Microbiol. 2001 Jan.

Abstract

Salmonella enterica serovar Enteritidis was detected in artificially inoculated eggs within 24 h through a rapid monoclonal antibody-based dot blot immunoassay. Detection in poultry and other products required 28 h. Samples were directly enriched in homogenized egg without the need for pre- or postenrichment steps. Serovar Enteritidis was detected in the presence of other bacteria when outcompeted 1:400.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Detection of serovar Enteritidis in artificially inoculated eggs by the dot blot immunoassay. Values are the initial inocula (CFU) per 25 g of egg.
FIG. 2
FIG. 2
Detection of serovar Enteritidis in artificially inoculated eggs in the presence of a mixed population of bacteria (serovar Heidelberg, P. vulgaris, P. fluorescens, E. coli, C. freundii, and A. faecalis). Values are the ratios of serovar Enteritidis to the mixed population of bacteria per 50 g of egg.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Association of Official Analytical Chemists. Official methods of analysis. 16th ed. Arlington, Va: Association of Official Analytical Chemists; 1995. pp. 55–94.
    1. Baker R C. Survival of Salmonella enteritidis on and in shelled eggs, liquid eggs, and cooked egg products. Dairy Food Environ San. 1990;10:273–275.
    1. Barnhart H M, Dreesen D W, Bastien R, Pancorbo O C. Prevalence of Salmonella enteritidis and other serovars in ovaries of layer hens at time of slaughter. J Food Prot. 1991;54:488–491. - PubMed
    1. Blais B W, Yamazaki H. Use of detergents in the preparation of Salmonella samples for enzyme immunoassay on polymyxin-coated polyester cloth. Int J Food Microbiol. 1990;11:329–336. - PubMed
    1. Board R G. Review article: the course of microbial infection of the hen's egg. J Appl Bacteriol. 1966;29:319–341. - PubMed

Publication types

LinkOut - more resources