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. 2001 Jan;67(1):464-8.
doi: 10.1128/AEM.67.1.464-468.2001.

Surface interactions between Escherichia coli and hemocytes of the Mediterranean mussel Mytilus galloprovincialis lam. leading to efficient bacterial clearance

L Canesi  1 , C Pruzzo, R Tarsi, G Gallo
Affiliations

Surface interactions between Escherichia coli and hemocytes of the Mediterranean mussel Mytilus galloprovincialis lam. leading to efficient bacterial clearance

L Canesi et al. Appl Environ Microbiol. 2001 Jan.

Abstract

The role of type 1 fimbriae in the interactions between Escherichia coli and Mytilus galloprovincialis Lam. hemocytes was evaluated. The association of fimbriated strain MG155 with hemocyte monolayers at 18 degrees C was 1.5- and 3- to 4-fold greater than the association of unfimbriated mutant AAEC072 in artificial seawater and in hemolymph serum, respectively. Such differences were apparently due to different adhesive properties since MG155 adhered more efficiently than AAEC072 when hemocytes were incubated at 4 degrees C to inhibit the internalization process. Hemolymph serum increased both association and adherence of MG155 two- to threefold but did not affect association and adherence of AAEC072. MG155 was also 1.5- to 1.7-fold more sensitive to killing by hemocytes than AAEC072, as evaluated by the number of culturable bacteria after 60 and 120 min of incubation. The role of type 1 fimbriae in MG155 interactions with hemocytes was confirmed by the inhibitory effect of D-mannose. In in vivo experiments MG155 cells were cleared from circulating hemolymph more rapidly than AAEC072 cells were cleared. These results confirm that surface properties are crucial in influencing bacterial persistence and survival within mussel hemolymph.

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Figures

FIG. 1
FIG. 1
Survival of fimbriated strain MG1655 and unfimbriated strain AAEC072 of E. coli in hemocyte monolayers in vitro (A) and in mussel hemolymph in vivo (B). (A) Number of culturable bacteria per milliliter in the hemocyte monolayer. Symbols: ■, MG1655; □, AAEC072; ●, MG1655 in the presence of d-galactose; ○, MG1655 in the presence of d-mannose. (B) Number of culturable bacteria per milliliter of hemolymph. In this experiment, zero time corresponded to 30 postinjection (that is, the time interval required for hemolymph to reach equilibrium after bacterial injection), as described in the text. Symbols: ■, MG1655; □, AAEC072. Bars indicate standard deviations.
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