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. 1975 Mar;15(3):466-70.
doi: 10.1128/JVI.15.3.466-470.1975.

Method for determination of nucleotide sequence homology between viral genomes by DNA reassociation kinetics

K Fujinaga, K Sekikawa, H Yamazaki

Method for determination of nucleotide sequence homology between viral genomes by DNA reassociation kinetics

K Fujinaga et al. J Virol. 1975 Mar.

Abstract

A model and appropriate equations were derived for the quantitative estimation of nucleotide sequence homology between two partially related viral genomes by measurement of the initial rate of reassociation of one labeled DNA in the presence of a second unlabeled DNA. The validity and usefulness of this procedure were demonstrated by the analysis of the reassociation kinetics of labeled adenovirus 7 DNA in the presence of unlabeled adenovirus 2 DNA. Based on DNA reassociation, the extent of homology between adenovirus 2 and 7 genomes was found to be 10 to 12%. The duplex formed between adenovirus 2 and 7 DNA had the appropriate thermal stability for a well-matched DNA-DNA hybrid.

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References

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