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. 2001 Jan;39(1):235-40.
doi: 10.1128/JCM.39.1.235-240.2001.

Limited genetic diversity of Brucella spp

B Gándara  1 , A L Merino, M A Rogel, E Martínez-Romero
Affiliations

Limited genetic diversity of Brucella spp

B Gándara et al. J Clin Microbiol. 2001 Jan.

Abstract

Multilocus enzyme electrophoresis (MLEE) of 99 Brucella isolates, including the type strains from all recognized species, revealed a very limited genetic diversity and supports the proposal of a monospecific genus. In MLEE-derived dendrograms, Brucella abortus and a marine Brucella sp. grouped into a single electrophoretic type related to Brucella neotomae and Brucella ovis. Brucella suis and Brucella canis formed another cluster linked to Brucella melitensis and related to Rhizobium tropici. The Brucella strains tested that were representatives of the six electrophoretic types had the same rRNA gene restriction fragment length polymorphism patterns and identical ribotypes. All 99 isolates had similar chromosome profiles as revealed by the Eckhardt procedure.

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Figures

FIG. 1
FIG. 1
Dendrogram derived from MLEE with 10 enzymes analyzed: isocitrate dehydrogenase, malate dehydrogenase, glucose-6-phosphate dehydrogenase, NADP-dependent glutamate dehydrogenase, pyruvate dehydrogenase, indophenol oxidase, hexokinase, aconitase, phosphoglucomutase, and phosphoglucose isomerase.
FIG. 2
FIG. 2
Dendrogram derived from MLEE with 16 enzymes analyzed (see Materials and Methods).
FIG. 3
FIG. 3
Plasmid megaplasmids and chromosomes as visualized by the modified Eckhardt procedure. Lanes: 1, B. abortus bv. 1; 2, B. melitensis bv. 1; 3, B. abortus bv. 4; 4, B. suis bv. 3; 5, B. suis bv. 4; 6, B. suis bv. 5; 7, R. etli CFN42; 8, R. meliloti 1021; 9, R. tropici type A reference strain CFN299.
See this image and copyright information in PMC

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