Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2001 Jan;39(1):328-31.
doi: 10.1128/JCM.39.1.328-331.2001.

Binary typing of Staphylococcus aureus strains through reversed hybridization using digoxigenin-universal linkage system-labeled bacterial genomic DNA

W van Leeuwen  1 , C Libregts, M Schalk, J Veuskens, H Verbrugh, A van Belkum
Affiliations
Comparative Study

Binary typing of Staphylococcus aureus strains through reversed hybridization using digoxigenin-universal linkage system-labeled bacterial genomic DNA

W van Leeuwen et al. J Clin Microbiol. 2001 Jan.

Abstract

A novel binary typing (BT) procedure, based on reversed hybridization of digoxigenin-universal linkage system-labeled bacterial DNA to strip-immobilized probes, is presented. Chromogenic detection of hybrids was performed. Staphylococcus aureus isolates (n = 20) were analyzed to establish the feasibility of BT. A technically simple and fast procedure has been developed for application in routine microbiology laboratories.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Schematic diagram showing the novel binary typing protocol. hyb., hybridization.
FIG. 2
FIG. 2
Binary typing results for the methicillin-resistant S. aureus strain collection obtained after hybridization on the strip-immobilized DNA probe panel (probes AW-1 through AW-15).
See this image and copyright information in PMC

References

    1. Allerberger F, Fritschel S J. Use of automated ribotyping of Austrian Listeria monocytogenes isolates to support epidemiological typing. J Microbiol Methods. 1999;35:237–244. - PubMed
    1. Anonymous. Preparation of genomic DNA from bacteria. In: Ausubel R B F M, Kingston R E, Moore D D, Seidman J G, Smith J A, Struhl K, editors. Current protocols in molecular biology. Vol. 1. New York, N.Y: John Wiley & Sons, Inc.; 1994. pp. 2.4.1–2.4.2.
    1. Boom R, Sol C J, Salimans M M, Jansen C L, Wertheim-van Dillen P M, van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol. 1990;28:495–503. - PMC - PubMed
    1. Kamerbeek J, Schouls L, Kolk A, van Agterveld M, van Soolingen D, Kuijper S, Bunschoten A, Molhuizen H, Shaw R, Goyal M, van Embden J. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol. 1997;35:907–914. - PMC - PubMed
    1. Sambrook J E, Fritsch E F, Maniatis T. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory Press; 1989.

Publication types

MeSH terms