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Case Reports
. 2001 Jan;39(1):365-6.
doi: 10.1128/JCM.39.1.365-366.2001.

Isolation of Legionella anisa using an amoebic coculture procedure

B La Scola  1 , L Mezi, P J Weiller, D Raoult
Affiliations
Case Reports

Isolation of Legionella anisa using an amoebic coculture procedure

B La Scola et al. J Clin Microbiol. 2001 Jan.

Abstract

Conventional diagnostic tests for legionellosis were negative for a 61-year-old immunocompromised man with pneumonia. However, coculture of a sputum sample with Acanthamoeba polyphaga amoebae led to the recovery of Legionella anisa. This procedure may be a sensitive and convenient diagnostic method, especially for non-Legionella pneumophila species infections that can be diagnosed only by culture.

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Figures

FIG. 1
FIG. 1
Giemsa-stained aggregates (arrowheads) of L. anisa within A. polyphaga amoebae (magnification, ×1,000).
See this image and copyright information in PMC

References

    1. Birtles R J, Rowbotham T J, Raoult D, Harrison T G. Phylogenetic diversity of intra-amoebal legionellae as revealed by 16S rRNA gene sequence comparison. Microbiology. 1996;142:3525–3530. - PubMed
    1. Bornstein N, Mercatello A, Marmet D, Surgot M, Deveaux Y, Fleurette J. Pleural infection caused by Legionella anisa. J Clin Microbiol. 1989;27:2100–2101. - PMC - PubMed
    1. Edelstein P H. Legionnaires' disease. Clin Infect Dis. 1993;16:741–747. - PubMed
    1. Fallon R J, Stack B H R. Legionnaires' disease due to Legionella anisa. J Infect. 1990;20:227–229. - PubMed
    1. Fenstersheib M D, Miller M, Diggins C, Liska S, Detwiler L, Benson Werner S, Lindquist D, Lanier Thacker W, Benson R. Outbreak of Pontiac fever due to Legionella anisa. Lancet. 1990;336:35–37. - PubMed

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