Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Case Reports
. 2001 Jan;39(1):365-6.
doi: 10.1128/JCM.39.1.365-366.2001.

Isolation of Legionella anisa using an amoebic coculture procedure

B La Scola  1 , L Mezi, P J Weiller, D Raoult
Affiliations
Case Reports

Isolation of Legionella anisa using an amoebic coculture procedure

B La Scola et al. J Clin Microbiol. 2001 Jan.

Abstract

Conventional diagnostic tests for legionellosis were negative for a 61-year-old immunocompromised man with pneumonia. However, coculture of a sputum sample with Acanthamoeba polyphaga amoebae led to the recovery of Legionella anisa. This procedure may be a sensitive and convenient diagnostic method, especially for non-Legionella pneumophila species infections that can be diagnosed only by culture.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Giemsa-stained aggregates (arrowheads) of L. anisa within A. polyphaga amoebae (magnification, ×1,000).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Birtles R J, Rowbotham T J, Raoult D, Harrison T G. Phylogenetic diversity of intra-amoebal legionellae as revealed by 16S rRNA gene sequence comparison. Microbiology. 1996;142:3525–3530. - PubMed
    1. Bornstein N, Mercatello A, Marmet D, Surgot M, Deveaux Y, Fleurette J. Pleural infection caused by Legionella anisa. J Clin Microbiol. 1989;27:2100–2101. - PMC - PubMed
    1. Edelstein P H. Legionnaires' disease. Clin Infect Dis. 1993;16:741–747. - PubMed
    1. Fallon R J, Stack B H R. Legionnaires' disease due to Legionella anisa. J Infect. 1990;20:227–229. - PubMed
    1. Fenstersheib M D, Miller M, Diggins C, Liska S, Detwiler L, Benson Werner S, Lindquist D, Lanier Thacker W, Benson R. Outbreak of Pontiac fever due to Legionella anisa. Lancet. 1990;336:35–37. - PubMed

Publication types

LinkOut - more resources