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. 2001 Jan;39(1):401-3.
doi: 10.1128/JCM.39.1.401-403.2001.

Extraction and genotyping of Cryptosporidium parvum DNA from fecal smears on glass slides stained conventionally for direct microscope examination

C Amar  1 , S Pedraza-Díaz, J McLauchlin
Affiliations

Extraction and genotyping of Cryptosporidium parvum DNA from fecal smears on glass slides stained conventionally for direct microscope examination

C Amar et al. J Clin Microbiol. 2001 Jan.

Abstract

A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of Cryptosporidium parvum was amplified by PCR from 89 (85%) of 105 smears following conventional staining but not from negative controls. This technique may have applications for analysis of other infectious agents.

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References

    1. Anonymous. PHLS Standard Operating Procedure, B.SOP 30. Investigation of faeces specimens. London, United Kingdom: Public Health Laboratory Service; 1999.
    1. Arrowood M J. Diagnosis. In: Fayer R, editor. Cryptosporidium and cryptosporidiosis. Boca Raton, Fla: CRC Press, Inc.; 1997. pp. 43–64.
    1. Boom R, Sol C J A, Salimans M M M, Jansen C L, Wertheim-van Dillen P M E, van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol. 1990;28:495–503. - PMC - PubMed
    1. Casemore D P, Armstrong M, Sands R L. Laboratory diagnosis of cryptosporidiosis. J Clin Pathol. 1985;38:1337–1341. - PMC - PubMed
    1. Fayer R, Speer C A, Dubey J P. The general biology of Cryptosporidium. In: Fayer R, editor. Cryptosporidium and cryptosporidiosis. Boca Raton, Fla: CRC Press, Inc.; 1997. pp. 1–41.

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