Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Dec;38(4):245-9.
doi: 10.3347/kjp.2000.38.4.245.

Detection of IgG antibody against Neospora caninum in cattle in Korea

J S Bae  1 , D Y Kim, W S Hwang, J H Kim, N S Lee, H W Nam
Affiliations

Detection of IgG antibody against Neospora caninum in cattle in Korea

J S Bae et al. Korean J Parasitol. 2000 Dec.

Abstract

A total of 492 cattle sera was screened by IgG-ELISA against Neospora caninum (Nc-1 strain and a Korean isolate, KBA-2) and Toxoplasma gondii. Out of 492, 113 sera (23.0%) reacted positively to either Nc-1 or KBA-2 strains of N. caninum. Among the 113 positive sera, 92 sera (81.4%) reacted with antigens of both strains, but 6 sera (5.3%) with Nc-1 and 15 sera (13.3%) with KBA-2 strain only. And with T. gondii antigen, 6 sera (1.2%) were positive but all reacted with N. caninum antigen also. Western blot revealed typical binding pattern according to ELISA values, such that high OD group reacted specifically to the major surface proteins including 43 kDa protein. Seroprevalence of 23.0% indicates that neosporosis seemed to be one of major causes of abortion in cattle. It is suggested here to establish more epidemiological researches nationwide systematically.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Distribution of positive OD spots of Neospora caninum (Nc-1 and KBA-2 strains) vs. Toxoplasma gondii by IgG-ELISA.
Fig. 2
Fig. 2
Distribution of positive OD spots of Neospora caninum KBA-2 vs. Nc-1 strain.
Fig. 3
Fig. 3
Western blot of sera of various OD values by ELISA. N, Nc-1; K, KBA-2 strain of Neospora caninum; T, Toxoplasma gondii. A, positive with both Nc-1 and KBA-2 antigens; B, positive with both but much higher with KBA-2; C, Nc-1 negative but KBA-2 positive; D, Nc-1 positive but KBA-2 negative; E, positive with Nc-1, KBA-2, and T. gondii.
See this image and copyright information in PMC

References

    1. Atkinson R, Harper PA, Ryce C, Morrison DA, Ellis JT. Comparison of the biological characteristics of two isolates of Neospora caninum. Parasitology. 1999;118:363–370. - PubMed
    1. Bjerkas I, Jenkins MC, Dubey JP. Identification and characterization of Neospora caninum tachyzoite antigens useful for diagnosis of neosporosis. Clin Diagn Lab Immunol. 1994;1:214–221. - PMC - PubMed
    1. Brindley PJ, Gazzinelli RT, Denkers SW, et al. Differentiation of Toxoplasma gondii from closely related coccidia by riboprint analysis and a surface antigen gene polymerase chain reaction. Am J Trop Med Hyg. 1993;48:447–456. - PubMed
    1. Choi WY, Nam HW, Youn JH, et al. Detection of antibodies in serum and cerebrospinal fluid to Toxoplasma gondii by indirect latex agglutination test and enzyme-linked immunosorbent assay. Korean J Parasitol. 1992;30:83–90. - PubMed
    1. Davison HC, Otter A, Trees AJ. Significance of Neospora caninum in British dairy cattle determined by estimation of seroprevalence in normally calving cattle and aborting cattle. Int J Parasitol. 1999;29:1189–1194. - PubMed

Publication types

LinkOut - more resources