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. 2000 Dec;38(4):245-9.
doi: 10.3347/kjp.2000.38.4.245.

Detection of IgG antibody against Neospora caninum in cattle in Korea

J S Bae  1 , D Y Kim, W S Hwang, J H Kim, N S Lee, H W Nam
Affiliations

Detection of IgG antibody against Neospora caninum in cattle in Korea

J S Bae et al. Korean J Parasitol. 2000 Dec.

Abstract

A total of 492 cattle sera was screened by IgG-ELISA against Neospora caninum (Nc-1 strain and a Korean isolate, KBA-2) and Toxoplasma gondii. Out of 492, 113 sera (23.0%) reacted positively to either Nc-1 or KBA-2 strains of N. caninum. Among the 113 positive sera, 92 sera (81.4%) reacted with antigens of both strains, but 6 sera (5.3%) with Nc-1 and 15 sera (13.3%) with KBA-2 strain only. And with T. gondii antigen, 6 sera (1.2%) were positive but all reacted with N. caninum antigen also. Western blot revealed typical binding pattern according to ELISA values, such that high OD group reacted specifically to the major surface proteins including 43 kDa protein. Seroprevalence of 23.0% indicates that neosporosis seemed to be one of major causes of abortion in cattle. It is suggested here to establish more epidemiological researches nationwide systematically.

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Figures

Fig. 1
Fig. 1
Distribution of positive OD spots of Neospora caninum (Nc-1 and KBA-2 strains) vs. Toxoplasma gondii by IgG-ELISA.
Fig. 2
Fig. 2
Distribution of positive OD spots of Neospora caninum KBA-2 vs. Nc-1 strain.
Fig. 3
Fig. 3
Western blot of sera of various OD values by ELISA. N, Nc-1; K, KBA-2 strain of Neospora caninum; T, Toxoplasma gondii. A, positive with both Nc-1 and KBA-2 antigens; B, positive with both but much higher with KBA-2; C, Nc-1 negative but KBA-2 positive; D, Nc-1 positive but KBA-2 negative; E, positive with Nc-1, KBA-2, and T. gondii.
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References

    1. Atkinson R, Harper PA, Ryce C, Morrison DA, Ellis JT. Comparison of the biological characteristics of two isolates of Neospora caninum. Parasitology. 1999;118:363–370. - PubMed
    1. Bjerkas I, Jenkins MC, Dubey JP. Identification and characterization of Neospora caninum tachyzoite antigens useful for diagnosis of neosporosis. Clin Diagn Lab Immunol. 1994;1:214–221. - PMC - PubMed
    1. Brindley PJ, Gazzinelli RT, Denkers SW, et al. Differentiation of Toxoplasma gondii from closely related coccidia by riboprint analysis and a surface antigen gene polymerase chain reaction. Am J Trop Med Hyg. 1993;48:447–456. - PubMed
    1. Choi WY, Nam HW, Youn JH, et al. Detection of antibodies in serum and cerebrospinal fluid to Toxoplasma gondii by indirect latex agglutination test and enzyme-linked immunosorbent assay. Korean J Parasitol. 1992;30:83–90. - PubMed
    1. Davison HC, Otter A, Trees AJ. Significance of Neospora caninum in British dairy cattle determined by estimation of seroprevalence in normally calving cattle and aborting cattle. Int J Parasitol. 1999;29:1189–1194. - PubMed

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