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. 2000;14(6):280-3.
doi: 10.1002/1098-2825(20001212)14:6<280::AID-JCLA6>3.0.CO;2-0.

Modified salting-out method for DNA isolation from newborn cord blood nucleated cells

N I Noguera  1 , C E Tallano, I M Bragós, A C Milani
Affiliations

Modified salting-out method for DNA isolation from newborn cord blood nucleated cells

N I Noguera et al. J Clin Lab Anal. 2000.

Abstract

The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the beta-globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a considerable number of cord blood samples. By introducing a simple phenol/chloroform step, before ethanol precipitation of the nucleic acid, to certain samples, we were able to eliminate or substantially reduce the presence of inhibitors to PCR without having to re-extract the samples.

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