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. 2000;14(6):327-9.
doi: 10.1002/1098-2825(20001212)14:6<327::aid-jcla13>3.0.co;2-8.

Plasma beta-N-acetylhexosaminidase isoenzyme composition and temperature conversion factors

J A Casal  1 , L F Pérez, J C Tutor
Affiliations

Plasma beta-N-acetylhexosaminidase isoenzyme composition and temperature conversion factors

J A Casal et al. J Clin Lab Anal. 2000.

Abstract

Using the chromogenic substrate 3,3'-dichlorophenylsulfonphthaleinyl-N-acetyl-beta-D-glucosaminide for the activity determination of plasma beta-N-acetylhexosaminidase (Hex), the temperature conversion factors (TCF) offer a highly significant positive correlation with the relative proportion of Hex B isoenzyme (P< 0.001). The calculation of TCF 37 degrees/25 degrees C allows the isoenzyme composition of Hex to be determined quickly and cheaply. The results may be superimposed over those obtained in a previously described method based on the calculation of the enzyme's activation energy using four temperatures. However, the use of TCF 37 degrees/30 degrees C does not appear to comply with the required demands.

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