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. 2000 Dec;4(6):373-6.
doi: 10.1007/s007920070007.

Direct proof for the presence and expression of two 5% different 16S rRNA genes in individual cells of Haloarcula marismortui

G Amann  1 , K O Stetter, E Llobet-Brossa, R Amann, J Antón
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Direct proof for the presence and expression of two 5% different 16S rRNA genes in individual cells of Haloarcula marismortui

G Amann et al. Extremophiles. 2000 Dec.

Abstract

A single-cell-derived pure culture of Haloarcula marismortui was obtained using the optical tweezers technique. Analysis of the 16S rRNA genes unambiguously demonstrated two 5% different 16S rRNA sequences. In addition, expression of both operons in the same cell was shown by fluorescence in situ hybridization with operon-specific probes.

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