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. 2001 Jan;8(1):166-9.
doi: 10.1128/CDLI.8.1.166-169.2001.

Lateral-flow assay for rapid serodiagnosis of human leptospirosis

H L Smits  1 , C K Eapen, S Sugathan, M Kuriakose, M H Gasem, C Yersin, D Sasaki, B Pujianto, M Vestering, T H Abdoel, G C Gussenhoven
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Lateral-flow assay for rapid serodiagnosis of human leptospirosis

H L Smits et al. Clin Diagn Lab Immunol. 2001 Jan.

Abstract

An assay device for the rapid detection of Leptospira-specific immunoglobulin M (IgM) antibodies in human sera is presented. The sensitivity (85.8%) and specificity (93.6%) of the assay compared well (91.9% agreement) with those of an IgM enzyme-linked immunosorbent assay routinely used in the serodiagnosis of leptospirosis. The sensitivity of the assay varied with the stage of the disease. The assay uses stabilized components and is simply performed by the addition of serum and sample fluid to the sample well of the assay device. The assay is read after 10 min, and a positive result is obtained when staining of the test line is observed.

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