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. 2001 Jan;8(1):174-7.
doi: 10.1128/CDLI.8.1.174-177.2001.

Immunodiagnosis of human fascioliasis by an enzyme-linked immunosorbent assay (ELISA) and a micro-ELISA

S Carnevale  1 , M I Rodríguez, G Santillán, J H Labbé, M G Cabrera, E J Bellegarde, J N Velásquez, J E Trgovcic, E A Guarnera
Affiliations

Immunodiagnosis of human fascioliasis by an enzyme-linked immunosorbent assay (ELISA) and a micro-ELISA

S Carnevale et al. Clin Diagn Lab Immunol. 2001 Jan.

Abstract

Enzyme-linked immunosorbent assay (ELISA) and micro-ELISA were evaluated for their ability to detect anti-Fasciola hepatica antibodies in humans by using excretory-secretory antigen. The sensitivity of each method was 100%, but the specificity was 100% for ELISA and 97% for micro-ELISA. The micro-ELISA could be used as a screening assay and ELISA could be used as a confirmatory method for the serodiagnosis of human fascioliasis.

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Figures

FIG. 1
FIG. 1
ELISA absorbances of serum samples using FhESA. (A) Analysis of sera obtained from 100 negative control patients and 22 individuals parasitologically positive for F. hepatica infection. The y axis shows the frequency of absorbance measurements. The vertical dashed line represents the cutoff point, which was calculated as 3.09 SDs from the mean of the seronegative group. (B) Specificity of ELISA using sera from groups of patients with proven infections with Toxoplasma gondii (Tg), Trypanosoma cruzi (Tc), Leishmania spp. (Ls), Plasmodium vivax (Pv), cysticercosis (Ci), hydatid disease (Hi), trichinosis (Tq), toxocariasis (Tx), Schistosoma mansoni (Sm), Ascaris lumbricoides (Al), Ancylostoma duodenale (Ad), Enterobius vermicularis (Ev), Strongyloides stercoralis (Ss), Trichuris trichiura (Tt), Taenia spp. (Ts), Entamoeba histolytica (Eh), Giardia lamblia (Gl), syphilis (Sp), tuberculosis (Tb), anti-hepatitis A virus IgM (HAM), IgG antibodies against hepatitis A virus (HAG), hepatitis B (positive for surface antigen) (HB), and hepatitis C (HC). The dashed horizontal line represents the cutoff point. The individuals are indicated by closed squares.
FIG. 2
FIG. 2
Histogram showing the analysis of positive and negative sera by micro-ELISA with FhESA. The dashed line represents the calculated cutoff point.
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